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| 产品名称： | NCI-H748 H748 |
|---|---|
| 商品货号： | TS172875 |
| Organism： | Homo sapiens, human |
| Tissue： | lung; Derived from metastatic site: lymph node |
| Product Format： | flask |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | stage E, carcinoma; classic small cell lung cancer |
| Age： | 62 years |
| Gender： | male |
| Ethnicity： | Black |
| Karyotype： | t(1;3)del(3)(p14-p23) |
| Derivation： | The line was established in July 1984. |
| Clinical Data： | male
Black
62 years |
| Comments： | The line was established in July 1984.
The patient received prior chemotherapy and radiation therapy.
The patient was a smoker. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) Cultures can be maintained by addition of fresh medium. Cells grow as floating aggregates of round cell clusters. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 12 D5S818: 10 D7S820: 10,11 THO1: 7,9 TPOX: 10,12 vWA: 17,19 |
| Name of Depositor： | AF Gazdar, JD Minna |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1984 |
| References： | NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996. |