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微信小章| 产品名称： | Enterovirus D68 |
|---|---|
| 商品货号： | TS172952 |
| Classification： | Picornaviridae, Enterovirus, Enterovirus D |
| Deposited As： | Enterovirus 68 |
| Agent： | Enterovirus D68 |
| Strain： | Fermon |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL per vial |
| Storage Conditions： | -70°C or colder |
| Genome Sequenced Strain： | Human enterovirus 68 strain Fermon, complete genome |
| Images： |  |
| Comments： | ATCC® VR-561™ was depositor material produced in primary monkey kidney cells. ATCC®xa0VR-1826™ was created by serially passaging ATCC®xa0VR-561™ four times onto MRC-5 (ATCC® CCL-171™) cells. |
| Effect on Host： | CPE in cell culture |
| Recommended Host： | MRC-5 (ATCC® CCL-171™) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation:xa02-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | EH Lennette |
| Source： | Isolated from a nasal-pharyngeal swab of a hospitalized 10-month-old female with pneumonia, California, 1962 |
| References： | Schieble JH, et al. A probable new human picornavirus associated with respiratory diseases. Am. J. Epidemiol. 85: 297-310, 1967. PubMed: 4960233 |
| Cross References： | Nucleotide (GenBank) : AY426531.1 Human enterovirus 68 strain Fermon, complete genome |