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| 产品名称： | pYNM18.1 |
|---|---|
| 商品货号： | TS172986 |
| Designations： | pYNM18.1 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | Codominant segregation of the TaqI RFLP was observed in 55 three-generation families. Otherwise, RFLPs were observed under normal hybridization and wash stringencies. |
| Vector： | Construct size (kb): 7.0 |
| Insert： | DNA: genomic Insert lengths(kb): 4.300000190734863 Gene product: DNA Segment, single copy D15S35 Alleles: B1, B2, A1, A2, A3 |
| Insert Size (kb)： | 4.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--7.0; PstI--4.3, 2.7; HindIII--4.3, 2.7; EcoRI/HindIII--2.7, 2.2 (doublet); BamHI--7.0; consistent with deposited DNA. The structure of this plasmid is not consistent with the published description of pYNM18.1. The hybridization pattern of this probe with human DNA was not checked by the ATCC. Codominant segregation of the TaqI RFLP was observed in 55 three-generation families. This probe was pre-associated with excess human DNA prior to hybridization. Otherwise, RFLPs were observed under normal hybridization and wash stringencies. Enzyme(s) not detecting polymorphism: RsaI, BglII, PvuII and PstI. |
| References： | Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pYNM18.1) on chromosome 15 D15S35. Nucleic Acids Res. 16: 3592, 1988. PubMed: 2897661 |