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| 产品名称： | pSON8 |
|---|---|
| 商品货号： | TS172988 |
| Designations： | pSON8 |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.4689998626708980 Vector: pSON8 (plasmid) Promoters: Promoter ADH Construction: pUC Marker(s):URA3,ampR Construct size (kb): 9.468999862670898 Features: marker(s): URA3 marker(s): ampR promoter: ADH replicon: 2 micron ori replicon: pMB1 MCS: NcoI...EagI terminator: ADH coding sequence: SOS (human Son of sevenless) |
| Applications： | vector component of two-hybrid screening system |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--6.0, 3.5; XbaI--9.5; BamHI--9.5. SOS recruitment system (SRS) is a genetic screening method to detect proteins interacting in the cytoplasm. It is based on membrane targeting with a myristoylation signal and SOS-based activation of Ras protein. The vector contains the human SOS gene with a polylinker on the N-terminus to attach "bait" gene for use in SOS Recruitment System. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Aronheim A, et al. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. Mol. Cell. Biol. 17: 3094-3102, 1997. PubMed: 9154808 |
| Shipped： | freeze-dried |