| 产品名称: | RKShIIIA RKShIIIA.1 |
|---|---|
| 商品货号: | TS173024 |
| Designations: | RKShIIIA RKShIIIA.1 |
| GenBank Number: | M34052 |
| Species: | Rattus norvegicus, rat |
| Depositors: | B Rudy |
| Vector: | Construct size (kb): 6.019999980926514 |
| Insert: | DNA: cDNA Insert lengths(kb): 3.059999942779541 Tissue: brain Gene product: potassium channel, type III, Shaker homolog KCN3 |
| Insert Size (kb): | 3.060 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | Restriction digests of the clone give the following sizes (kb): XbaI--3.5, 2.5; PstI--5.3, 0.4, 0.2; EcoRI--2.8, 2.7, 0.4; HindIII--3.7, 1.0, 0.8, 0.3, 0.15; BamHI--4.9, 1.0, 0.18. The insert contains the following restriction sites (nt position according to the published sequence): PstI--159, 251, 630; EcoRI--300; PvuI--311; EcoRV--498; HindIII--754, 1077, 2030, 2280; BamHI--833, 1060; BglII--1828; SalI--2091; XbaI--2480. The total coding sequence is in an approximately 2.5 kb XbaI fragment. The sequence has been updated and corrected. |
| References: | McCormack T, et al. Molecular cloning of a member of a third class of Shaker-family K+ channel genes in mammals. Proc. Natl. Acad. Sci. USA 87: 5227-5231, 1990. PubMed: 2367536 McCormack K, et al. Shaker K+ channel subunits form heteromultimeric channels with novel functional properties. Biochem. Biophys. Res. Commun. 171: 1361-1371, 1990. PubMed: 1699527 McCormack T, et al. Molecular cloning of a member of a third class of Shaker-family K+ channel genes in mammals. Proc. Natl. Acad. Sci. USA 88: 4060, 1991. PubMed: 2023956 B Rudy, personal communication |