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| 产品名称： | pJ3omega |
|---|---|
| 商品货号： | TS173040 |
| Designations： | pJ3omega |
| Depositors： | H Land |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.5000000000000000 Vector: pJ3omega (plasmid) Promoters: Promoter SV40 early Construction: pBR322, SV40 Marker(s):ampR Construct size (kb): 3.5 Features: marker(s): ampR other: SV40 t IVS promoter: SV40 early replicon: SV40 replicon: pMB1 MCS: HindIII...BglII terminator: SV40 |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.5; HindIII--3.5; BamHI--3.5; SmaI--3.5; PvuI--2.15, 2.35. A shuttle vector for expressing sequences cloned into the polylinker site under the control of the SV40 promoter. Constructed from pBR322 (2066-4286, bla, ori), SV40 (4038/4648, t antigen IVS), SV40 (2461-2704, T antigen polyadenylation site), and SV40 (270-5171, early promoter, ori). |
| Media： | ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Morgenstern JP, Land H. A series of mammalian expression vectors and characterisation of their expression of a reporter gene in stably and transiently transfected cells. Nucleic Acids Res. 18: 1068, 1990. PubMed: 2156225 Hartmut Land, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |