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| 产品名称： | 10C4.1.3 |
|---|---|
| 商品货号： | TS173049 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain: BALB/c (B cell); BALB/c (myeloma) |
| Applications： | The antibody is specific for alpha v,beta 3 integrin. It recognizes an epitope on alpha v,beta 3 integrin that is specific to human osteoclasts. |
| Storage Conditions： | liqid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Derivation： | Animals were immunized with purified alpha v,beta 3 integrin. Spleen cells were fused with P3X63Ag8U.1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human alpha v,beta 3 integrin |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human alpha v,beta 3 integrin |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in pristane primed BALB/c mice |
| Comments： | Animals were immunized with purified alpha v,beta 3 integrin. Spleen cells were fused with P3X63Ag8U.1 myeloma cells. The antibody is specific for alpha v,beta 3 integrin. It recognizes an epitope on alpha v,beta 3 integrin that is specific to human osteoclasts. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml. Interval: Maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with an additional 10% fetal bovine serum and 7.5% (v/v) DMSO Storage temperature: liqid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | IgG1 kappa , IgG1; kappa light chain |
| Name of Depositor： | Genentech, Inc. |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Kim KJ, et al. Antibody to osteoclast alphavbeta3 ntegrin. US Patent 5,578,704 dated Nov 26 1996 |