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| 产品名称： | pCMV-His-ACS |
|---|---|
| 商品货号： | TS173067 |
| Designations： | pCMV-His-ACS |
| GenBank Number： | AF263614 |
| Species： | Homo sapiens, human |
| Depositors： | A Luong, JL Goldstein |
| Applications： | contains sequence acetyl-CoA synthetase |
| Vector： | Construct size (kb): 7.599999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pcDNA3.1(-) Intact vector size: 5.400 Type of vector: phagemid Vector end: BamHI Vector end: EcoRI Cloning sites: NheI ApaI XbaI XhoI NotI EcoRV EcoRI BamHI Asp718I KpnI HindIII AflII Polylinker sites: NheI PmeI ApaI XbaI XhoI NotI BstXI EcoRV EcoRI BstXI BamHI Asp718I KpnI HindIII AflII PmeI Host range: Escherichia coli Features (with orientation and position when available): promoter for expression: CMV, ->, 209-863 promoter for in vitro transcription: T7, ->, 863-882 MCS: NheI...HindIII, ->, 895-110 primer site: pcDNA3.1 reverse primer, terminator: bGH polyadenylation, ->, 1021-1235 replicon: f1, ->, 1298-1711 replicon: SV40, ->, 1776-2055 promoter: SV40, ->, 2056-2101 marker(s): G418R, ->, 2137-2931 terminator: SV40 polyadenylation, ->, 2986-3358 replicon: pMB1, 3618-4291 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CMV-His-ACS Gene name: acetyl-CoA synthetase Contains complete coding sequence?: Y Tissue: human Type of DNA: cDNA Insert end: BamHI Insert end: EcoRI Insert size (kb): 2.2 Cross references: Insert lengths(kb): 2.200000047683716 Tissue: human Gene product: acetyl-CoA synthetase CMV-His-ACS Target Gene: acetyl-CoA synthetase |
| Insert Size (kb)： | 2.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/NotI--5.4,2.2; BamHI--7.6; HindIII--6.85,0.45,0.3; XhoI--7.6. Encodes a 731-amino acid fusion protein that contains the full length human ACS. Consists of an initiator methionine, six consecutive histidines, an anti-Xpress antibody epitope (DLYDDDDK), and aa 2-701 of human ACS. Expression is driven by the CMV promoter/enhancer in the vector. |
| References： | Luong A, et al. Molecular characterization of human acetyl-CoA synthetase, an enzyme regulated by sterol regulatory element-binding proteins. J. Biol. Chem. 275: 26458-26466, 2000. PubMed: 10843999 |