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| 产品名称： | pWE1 |
|---|---|
| 商品货号： | TS173109 |
| Designations： | pWE1 |
| Depositors： | M Cockett |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.8590002059936520 DESCRIPTION OF VECTOR: Intact vector size: 6.859 Type of vector: phagemid Cloning sites: HindIII BspMI PstI XbaI BamHI EcoRI Polylinker sites: HindIII BspMI PstI SalI AccI XbaI BamHI AvaI XmaI SmaI EcoRI ApaI Host range: mammalian cells; Escherichia coli Features (with orientation and position when available): MCS: HindIII...ApaI, ->, 1-63 promoter for in vitro transcription: SP6, terminator: SV40 polyadenylation enhancer: SV40, 1099-1242 replicon: SV40, 1266-1401 marker(s): mycophenolic acid resistance (GPT), ->, 1614-2072 replicon: pMB1, marker(s): ampR, replicon: f1 ori, ->, 5385-5841 promoter for expression: CMV, ->, 6203-6790 promoter for in vitro transcription: T7, ->, 6834-6853 Vector: pWE1 (phagemid) Promoters: Promoter for in vitro transcription SP6 Marker(s):ampR,GPT Construct size (kb): 6.859000205993652 Features: marker(s): ampR marker(s): mycophenolic acid resistance (GPT) promoter for expression: CMV promoter for in vitro transcription: SP6 promoter for in vitro transcription: T7 replicon: SV40 replicon: f1 ori replicon: pMB1 MCS: HindIII...ApaI terminator: SV40 polyadenylation enhancer: SV40 |
| Applications： | expression vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; SalI--3.7, 3.3. Having identical cloning sites also facilitates shuttling cDNAs from one vector to another. One of a series of vectors (TS173109 - 87674) that allows simultaneous, high-level expression of multiple cDNA sequences in mammalian cells. A kit of all four vectors is also available (ATCC 87678). Each cDNA inserted into one of this series of vectors is regulated by an identical transcription unit, but each vector permits different selection to be applied to different subunits. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Cockett MI, et al. Simultaneous expression of multi-subunit proteins in mammalian cells using a convenient set of mammalian cell expression vectors. BioTechniques 23: 402-407, 1997. PubMed: 9298206 |
| Related Products： | component of:ATCC 87678 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |