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购买须知
| 产品名称： | ASK Atlantic Salmon Kidney |
|---|---|
| 商品货号： | TS173270 |
| Organism： | Salmo salar, salmon, Atlantic |
| Tissue： | kidney |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | female |
| Applications： | The ASK (Atlantic Salmon Kidney) cell line was derived by B. Krossoey in 1998 from cells taken from the normal kidney of an Atlantic Salmon. The cell line may be used to propagate Infectious Salmon Anemia virus (ISAV) of the Atlantic Salmon. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | xad |
| Clinical Data： | female |
| Comments： | The ASK (Atlantic Salmon Kidney) cell line was derived by B. Krossoey in 1998 from cells taken from the normal kidney of an Atlantic Salmon. The cell line may be used to propagate Infectious Salmon Anemia virus (ISAV) of the Atlantic Salmon. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Interval: Subculture every 10 to 17 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
Medium Renewal: Once per week. |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 100% Temperature: 20.0°C |
| Name of Depositor： | J Rolland, J Winton, B Krossoey |
| Deposited As： | Salmo salar |
| Year of Origin： | 1998 |
| References： | Devold M, et al. Use of RT-PCR for diagnosis of infectious salmon anaemia virus (ISAV) in carrier sea trout Salmo trutta after experimental infection. Dis. Aquat. Org. 40: 9-18, 2000. PubMed: 10785858 |