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微信小章| 产品名称： | pKKPAH.T87S |
|---|---|
| 商品货号： | TS173313 |
| Designations： | pKKPAH.T87S |
| GenBank Number： | M12337 |
| Species： | Rattus norvegicus, rat |
| Depositors： | JP Caradonna |
| Applications： | in suitable host, produces protein phenylalanine hydroxylase |
| Vector： | Construct size (kb): 4.796999931335449 |
| Insert： | DNA: Synthetic Insert lengths(kb): 1.368000030517578 Gene product: phenylalanine hydroxylase PAH Target Gene: phenylalanine hydroxylase |
| Insert Size (kb)： | 1.368 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.8, SalI--4.8; EcoRI/SalI--3.5, 1.4. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRV--0.17; NcoI--0.51; NdeI--1.02; PstI--0.70, 1.34; PvuII--0.01, 0.68; StuI--0.58; XhoI--0.75. Constructed by amplifying a product from the clone pRPH3. This contains a missense mutation (ACG->AGC) with respect to the GenBank sequence (M12337), resulting in serine at codon 87 rather than threonine. |
| References： | Kappock TJ, et al. Spectroscopic and kinetic properties of unphosphorylated rat hepatic phenylalanine hydroxylase expressed in Escherichia coli. J. Biol. Chem. 270: 30532-30544, 1995. PubMed: 8530485 Dahl HH, Mercer JF. Isolation and sequence of a cDNA clone which contains the complete coding region of rat phenylalanine hydroxylase. Structural homology with tyrosine hydroxylase, glucocorticoid regulation, and use of alternate polyadenylation sites. J. Biol. Chem. 261: 4148-4153, 1986. PubMed: 2869038 |