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| 产品名称： | phTGFB-2 |
|---|---|
| 商品货号： | TS173418 |
| Designations： | phTGFB-2 |
| Species： | Homo sapiens, human |
| Depositors： | GI Bell |
| Vector： | Construct size (kb): 5.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.138000011444092 Gene product: transforming growth factor, beta 1 TGFB Alleles: A1, A2, A1, A2 |
| Insert Size (kb)： | 2.138 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert includes the following restriction sites (in bp from the 5 end): XhoI (671), PstI (711), and BamHI (1718). Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.0; BamHI--3.2, 2.0; PstI--3.2, 2.0; XhoI--5.2; SalI--5.2. Contains the complete coding sequence of TGFB1, including 779 bp 5 untranslated sequence, 179 bp 3 untranslated sequence, and a poly(A) tract of 37 bp. Based on this, it appears to correspond roughly to nt 55-2200 of GenBank X02812. This probe detects a mRNA of 2.5 kb in peripheral blood mononuclear cells and LAK precursors. May cross hybridize with 28S ribosomal subunit. Enzyme(s) not detecting polymorphism: NruI, NsiI, PaeR7I, PstI, PvuII, RsaI, RsrII, SacI, SalI, Sau3AI, Sau96I, ScaI, SmaI, SpeI, SspI, StuI, StyI, TaqI, XbaI, XboI, XmnI (with DNA from 7 unrelated Caucasians). Enzyme(s) not detecting polymorphism: AvaII, BamHI, BanII, BclI, BglI, BglII, BstEI, BstNI, ClaI, Cfr13I, DraI, Eco0109, EcoRI, EcoRV, FokI, HaeII, HaeIII, HincII, HindIII, HinfI, HinpI, HpaI, HphI, KpnI, MboI, MboII, MspI, NciI, NdeI, NheI. |
| References： | Ardinger HH, et al. RFLP for the human transforming growth factor beta-1 gene on chromosome 19. Nucleic Acids Res. 16: 8202, 1988. PubMed: 2901721 Kasid A, et al. Effects of transforming growth factor-beta on human lymphokine-activated killer cell precursors. Autocrine inhibition of cellular proliferation and differentiation to immune killer cells. J. Immunol. 141: 690-698, 1988. PubMed: 3133414 Graeme I Bell, personal communication |