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| 产品名称： | rpPdc17 |
|---|---|
| 商品货号： | TS173483 |
| Designations： | rpPdc17 |
| GenBank Number： | M61147 |
| Species： | Rattus norvegicus, rat |
| Depositors： | C Craft |
| Vector： | Construct size (kb): 4.199999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.217000007629395 Tissue: pineal gland Gene product: phosducin(phosducin, pineal gland/retina) PDC Alleles: s, c |
| Insert Size (kb)： | 1.217 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.25; SacI--3.7, 0.6; HindIII--4.25; PstI--4.25. The insert contains the following restriction sites (nt from the 5 end): HindIII--75; SacI--640; EcoRV--786; ClaI--841. Contains the complete coding sequence (nt 55 - 795), plus 5 and 3 untranslated regions. Detects mRNA of approximately 1.3 kb in rat pineal and rat retina. The 5 end of the insert is closer to the KS primer and the T7 promoter. Transcription from the T7 promoter gives the sense strand. |
| References： | Craft CM, et al. Rat pineal gland phosducin: cDNA isolation, nucleotide sequence, and chromosomal assignment in the mouse. Genomics 10: 400-409, 1991. PubMed: 2071146 Cheryl Craft, personal communication |