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| 产品名称： | pHA10 |
|---|---|
| 商品货号： | TS173521 |
| Designations： | pHA10 |
| Depositors： | A Honigman |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 11.3900003433227500 Vector: pHA10 (plasmid) Promoters: Promoter none Construction: pBR322, lambdaEcoRI-D Marker(s):ampR Construct size (kb): 11.39000034332275 Features: insert detection: lambdakil marker(s): ampR promoter: none replicon: pMB1 repressor gene: cI857 |
| Applications： | vector permitting positive selection for inserts |
| Comments： | Temperature sensitive at 42C for viability. A positive selection vector for DNA inserts controlled by lambdaPL through the regulation of the kil gene. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Honigman A, et al. Plasmid vectors for positive selection of DNA inserts controlled by the lambda pL promoter, repressor and antitermination function. Gene 13: 289-298, 1981. PubMed: 6266919 |
| Shipped： | freeze-dried |