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| 产品名称： | pDK101 |
|---|---|
| 商品货号： | TS173542 |
| Designations： | pDK101 |
| Depositors： | B Weisblum |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.0269999504089360 Vector: pDK101 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pGEM-5Zf(+), synthetic adaptor Marker(s):ampR Construct size (kb): 3.026999950408936 Features: insert detection: lacZ marker(s): ampR promoter: lac promoter for in vitro transcription: SP6 promoter for in vitro transcription: T7 replicon: f1 replicon: pMB1 MCS: ApaI...NsiI |
| Applications： | vector permitting RNA synthesis in vitro vector permitting production of single-stranded DNA vector useful for cloning PCR products |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3; EcoRV--3.0; PstI--3.0. The plasmid contains the following restriction sites (bp from nt 1): BglI--1533, 2854; EcoRV--73; NcoI--37, 61; NdeI--105; NotI--85; PstI--93; PvuI--1786, 2885; PvuII--348, 2915; SacI--114; SalI--99; XcmI--44, 59; XmnI--2014. A high copy number vector useful for direct cloning of double stranded amplification products. Digestion of the vector with XcmI produces a linear molecule with 3 unpaired deoxythymidine residues at both ends, suitable for direct cloning of PCR products. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Kovalic D, et al. General method for direct cloning of DNA fragments generated by the polymerase chain reaction. Nucleic Acids Res. 19: 4560, 1991. PubMed: 1886782 |
| Shipped： | frozen |