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微信小章| 产品名称： | pRML1 |
|---|---|
| 商品货号： | TS173576 |
| Designations： | pRML1 |
| Depositors： | F Spencer |
| Biosafety Level： | 1 |
| Host： | Escherichia coli DH5α |
| Vector Information： | Size (kb): 9.6999998092651370
Vector: pRML1 (plasmid)
Promoters: Promoter for in vitro transcription T3
Construction: This plasmid was derived from pCGS966, and includes a TRP1 selectable marker, yeast origin of replication ARS1.
Marker(s): ampR
Cloning sites: BamHI NdeI SpeI ClaI SalI NotI EcoRI
Selectable marker cassette: TRP1, ← Replicon: ARS1,xa0← Insert detection: GAL, ← Centromere: CEN4, ← Promoter for in vitro transcription: T3, ← Replicon: ori, ← Marker: ampR, ← Coding sequence: TK, ← |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--9.7; EcoRI--9.7, BamHI/EcoRI--8.9, 0.8. The plasmid was derived from pCGS966, and includes a TRP1, ARS1, features for YAC amplification, a distal polylinker to support plasmid rescue of insert ends, T3 promoter, and a cloning polylinker for library production. Designed for use with pRML2. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Smith DR, et al. Amplification of large artificial chromosomes. Proc. Natl. Acad. Sci. USA 87: 8242-8246, 1990. PubMed: 2236036 Spencer F, et al. Targeted recombination-based cloning and manipulation of large DNA segments in yeast. Methods 5: 161-175, 1993. Smith DR, et al. Copy number amplification of yeast artifical chromosomes. Methods Enzymol. 216: 603-614, 1992. PubMed: 1336106 Zhong TP, et al. Zebrafish genomic library in yeast artifical chromosomes. Genomics 48: 136-138, 1998. PubMed: AMBIGUOUS 95 Haldi ML, et al. A comprehensive large-insert yeast artificial chromosome library for physical mapping of the mouse genome. Mamm. Genome 7: 767-769, 1996. PubMed: 8854865 |
| Shipped： | freeze-dried |
| Shipping Information： | in E. coli containing the plasmid |