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| 产品名称： | pPTGFbeta1 pTGFbeta33 |
|---|---|
| 商品货号： | TS173594 |
| Designations： | pPTGFbeta1 pTGFbeta33 |
| Species： | Sus scrofa, pig |
| Depositors： | S Qian |
| Applications： | The 0.7 kb SacI/PvuII fragment from within the coding region can be used as a hybridization probe. |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.299999952316284 Tissue: peripheral blood lymphocytes Gene product: transforming growth factor, beta 1 TGFB1 |
| Insert Size (kb)： | 2.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--3.05, 2.55; BamHI--3.2, 1.8, 0.65; KpnI--5.7; HindIII--4.0, 1.7; PvuII--5.7. The non-vector XhoI fragment contains the following restriction sites (approximate kb from the 5 end): BamHI--0.24, 0.84, 2.45; KpnI--0.92; HindIII--1.3; SacI--1.6; PuvII--0.05, 2.24, 2.28. A 1.7 kb BamHI fragment contains the entire coding region. Digestion with XhoI releases a 2.4 kb fragment that contains the insert plus approximately 200 bp of vector. The 0.7 kb SacI/PvuII fragment from within the coding region can be used as a hybridization probe. |
| References： | Kondaiah P, et al. cDNA cloning of porcine transforming growth factor-beta1 mRNAs; evidence for alternate splicing and polyadenylation. J. Biol. Chem. 263: 18313-18317, 1988. PubMed: 2461367 Su. Wen Qian, personal communication |