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微信小章| 产品名称： | pHPS9 |
|---|---|
| 商品货号： | TS173599 |
| Designations： | pHPS9 |
| Depositors： | S Bron |
| Applications： | shuttle vector |
| Vector： | Construct size (kb): 5.699999809265137 |
| Media： | ATCC Medium 1065 (see below) plus chloramphenicol (5.0 mcg/ml)
ATCC Medium 1065:
Tryptone (Difco 0123), 10.0 g
Yeast Extract (Difco 0127), 5.0 g
NaCl, 10.0 g
Distilled water, 1.0
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| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Plasmid was not verified by restriction analysis. Strain was checked for the following phenotypes: showed growth on LB + chloramphenicol (5 mcg/ml, but not 10 mcg/ml); blue colonies on IPTG + Xgal (80 mcg/ml); blue colonies on IPTG + Xgal (80 mcg/ml) + erythromycin (150 mcg/ml). Permits alpha-complementation to identify recombinants when used with Bacillus subtilis 6GM15, and alpha-complementation with plasmid marker rescue when used with Bacillis subtilis 6GM15pHPS9R (ATCC 37818). The cat86::lacZalpha fusion is in-frame. Expression is controlled by the P59 promoter from Lactococcus lactis subsp. cremoris Wg2. Cloning into the NdeI, NheI, BamHI, SmaI or EcoRI sites inactivates lacZalpha. The NcoI site interrupts the chloramphenicol resistance sequence. |
| References： | Haima P, et al. Development of a beta-galactosidase alpha-complementation system for molecular cloning in Bacillus subtilis. Gene 86: 63-69, 1990. PubMed: 2107125 Haima P, et al. An improved beta-galactosidase alpha-complementation system for molecular cloning in Bacillus subtilis. Gene 93: 41-47, 1990. PubMed: 2121609 Haima P, et al. Novel plasmid marker rescue transformation system for molecular cloning in Bacillus subtilis enabling direct selection of recombinants. Mol. Gen. Genet. 223: 185-191, 1990. PubMed: 2123518 |