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| 产品名称： | pSE640 |
|---|---|
| 商品货号： | TS173728 |
| Designations： | pSE640 |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 9.0000000000000000 Vector: pSE640 (plasmid) Construction: pUC Marker(s):URA3,ampR,SUP11 Construct size (kb): 9.0 Features: insert detection: lacZ marker(s): ampR, URA3, SUP11 replicon: pMB1, ARS1 centromere: CEN4 |
| Applications： | YC-type (centromeric) shuttle vector reporter construct shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; XhoI--9.2; SalI--9.2. YC-type shuttle enhancer-cloning vector using beta-galactosidase activity as the reporter. The UAS has been deleted from the CYC1 promoter, so expression of a CYC1-lacZ fusion protein depends on UAS elements cloned into the polylinker region. The order of the major features in this plasmid is: MCS - CYC - lacZ - pMB1 ori - ampR - URA3 - ARS1 - SUP11 - CEN4. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Stephen J Elledge, personal communication |
| Shipped： | freeze-dried |