微信小陈
微信小章| 产品名称： | pMCV1-H-J-I+V purified plasmid DNA |
|---|---|
| 商品货号： | TS173791 |
| Designations： | pMCV1-H-J-I+V purified plasmid DNA |
| GenBank Definition： | Molluscum contagiosum virus subtype 1, complete genome |
| GenBank Number： | U60315 |
| Depositors： | J Bugert |
| Vector： | Total size of construct (kb): 7.97 Marker: ampR Vector: pAT153 Vector type: cloning vector Insert site: HindIII Vector size (kb): 3.658 Markers: ampR Suggested primers for sequencing that flank HindIII site: Primer 1 (forward): 5’-AACCTATAAAAATAGGCGTATCACG-3’ Primer 2 (reverse): 5’-CGCATTGTTAGATTTCATACACGG-3’ |
| Insert： | Insert size (kb): 4.165 + 0.146 (4.311 kb total) Insert ends: HindIII DNA: partial genomic DNA Source: Molluscum contagiosum virus (MCV) Genbank accession: U60315, partial Nucleotides 1-4165 and 4166-4311 of the insert correspond to nucleotides 162560-166725 and 187252 – 187398 respectively, of Genbank U60315 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | 200 ng DNA |
| Comments： | This is a partial clone of the Molluscum contagiosum virus in a low copy number plasmid.xa0 The 4.2 and 0.146 kb insert includes nucleotides 162560-166725 and 187252-187398 of Genbank sequence U60315 for the complete genome of the virus. |
| References： | Bugert JJ, Darai G. Stability of molluscum contagiosum virus DNA among 184 patient isolates: evidence for variability of sequences in the terminal inverted repeats. J. Med. Virol. 33(3): 211-217, 1991. PubMed: 1880497 Melquiot NV, Bugert JJ. Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens. Methods Mol. Biol. 269: 371-384, 2004. PubMed: 15114026 Senkevich TG, et al. Genome sequence of a human tumorigenic poxvirus: prediction of specific host response-evasion genes. Science 273(5276): 813-816, 1996. PubMed: 8670425 |