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Plasmodium berghei Vincke and Lips

货号 TS173856
中文名称 null
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产品名称： Plasmodium berghei Vincke and Lips
商品货号： TS173856
Strain Designations： NK65D
Application：
Vector borne research
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
derived from M. Yoeli strain NK65 by mosquito passage, Univ. Illinois, Urbana, pre-1969
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Type Strain： no
Comments：
Malaria vaccine antigen
Isolation of a vaccine fraction
Variation in virulence
Malaria resistance
Cryopreservation：

Only young cells (rings) can be frozen in glycerolyte medium* because their membranes are more robust.

  1. To harvest parasites, inject host with ketamine (0.1-0.2 ml).
  2. Open chest cavity to expose heart and exsanguinate via cardiac puncture using Yaegers anticoagulant** (see below), 1 volume anticoagulant to 4 volumes blood.xa0 xa0 xa0
  3. Centrifuge blood for 5 mins. at 1800 rpm in 50 ml centrifuge tube.
  4. Aspirate supernatant using sterile Pasteur pipet.
  5. Resuspend pellet gently in remaining supernatant.
  6. Slowly add 5 volumes of glycerolyte medium to 3 volumes pellet dropwise via a syringe as follows:
    1. Add the first volume of glycerolyte and allow the tube to stand for 5 mins. at room temperature.
    2. Add the remaining 4 volumes of glycerolyte and gently agitate.
  7. Aliquot mixture into Nunc screw-capped freezing vials and place in a Nalgene 1°C cooling apparatus. Place the apparatus at -80°C overnight and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.).
  8. Plunge vials into liquid nitrogen (-196°C) the next day and store in liquid nitrogen or liquid nitrogen vapor.
Name of Depositor： NE Alger
Year of Origin： 1969
References：

Boyer JM, et al. Lipid composition and activity of a lytic factor isolated from Plasmodium berghei. Infect. Immun. 25: 805-809, 1979. PubMed: 387594

DAntonio LE, et al. Malaria vaccine antigen(s): detergent solubilization, partial isolation, and recovery of immunoprotective activity. Infect. Immun. 43: 442-444, 1984. PubMed: 6360908

Alger NE, et al. Plasmodium berghei NK65 in the inbred A-J mouse: variations in virulence of P. berghei demes. J. Protozool. 18: 598-601, 1971. PubMed: 5133123

Alger NE, Keshavarz-Valian H. Plasmodium berghei: the effects of suppressor factor on vaccination. Int. J. Parasitol. 14: 301-307, 1984. PubMed: 6381347

DAntonio LE, et al. Model system for study of artificially induced resistance to malaria. Nature 223: 507-509, 1969. PubMed: 5796953

DAntonio LE, et al. Malaria resistance: artificial induction with a partially purified plasmodial fraction. Science 168: 1117-1118, 1970. PubMed: 5441687

DAntonio LE. Plasmodium: a resume of the isolation of the vaccine fraction by the French pressure cell technique. Exp. Parasitol. 31: 75-81, 1972. PubMed: 5010607

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