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微信小章| 产品名称： | RW.4 |
|---|---|
| 商品货号： | TS173871 |
| Organism： | Mus musculus, mouse |
| Tissue： | inner cell mass |
| Cell Type： | embryonic stem cell |
| Product Format： | frozen |
| Morphology： | spherical colony |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Gender： | male |
| Strain： | 129X1/SvJ |
| Applications： | Gene knock-out - RW.4 cells have been used to create a number of different gene knock-out mice. Gene knock-in - RW.4 ES cells were used to create a knock-in mouse subline for use as a model of acute promyelocytic leukemia (APL). Differentiation - RW.4 ES cells can be induced by staurosporine (STS) to differentiate into neurons and astrocytes from embryoid bodies. ESC Proliferation - RW.4 cells have been used to study the effects of statins, cholesterol-lowering drugs, on ESC self-renewal, proliferation and differentiation. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | male |
| Comments： | The cells stain positive for pluripotency markers and alkaline phosphatase activity and RW.4 has been shown to be germline competent. |
| Complete Growth Medium： | Grow ES cells in Mouse ES Cell Basal Medium (ATCC SCRR-2011) that has been supplemented with the following components: 0.1 mM 2-mercaptoethanol (Invitrogen Cat. No. 21985) 1,000 U/ml mouse leukemia inhibitory factor (LIF) (EMD Millipore Cat. No. ESG1107) 15% FBS, ES Cell Qualified (ATCC SCRR-30-2020) Complete Growth Medium for Mouse ES Cells is stable for 14 days when stored at 2°C to 8°C. This medium is formulated for use with a 5% CO2 in air atmosphere. |
| Subculturing： | Subculturing Procedure Note: To insure the highest level of viability, pre-warm media and Trypsin/EDTA to 37ºC before adding to cells. Volumes used in this protocol are for T75 flasks. Proportionally adjust the volumes for culture vessels of other sizes. A split ratio of 1:4 to 1:7 is recommended. Feeder Cell Preparation for Subcultures
Dissociation and Transfer of ES Cells
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| Cryopreservation： | Freeze medium: Complete growth medium supplemented with an additional 10% FBS and 10% DMSO. Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | TJ Ley |
| Deposited As： | Mus musculus |
| References： | Hug BA, et al. Analysis of mice containing a targeted deletion of beta-globin locus control region 5 hypersensitive site 3. Mol. Cell. Biol. 16: 2906-2912, 1996. PubMed: 8649401 Matise MP, et al. Production of targeted Embryonic Stem Cell Clones. In AL Joyner (Ed.), Gene Targeting: A Practical Approach. Oxford University Press, Oxford, p. 101-132, 1999. Fabricius D, Bonde S, Zavazava N. Induction of stable mixed chimerism by embryonic stem cells requires functional Fas/FasL engagement. Transplantation 79(9): 1040-1044, 2005. PubMed: 15880040 Conte D, et al. Inhibitor of apoptosis protein cIAP2 is essential for lipopolysaccharide-induced macrophage survival. Mol. Cell Biol. 26(2): 699-708, 2006. PubMed: 16382159 Westervelt P, et al. High-penetrance mouse model of acute promyelocytic leukemia with very low levels of PML-RARalpha expression. Blood 102(5): 1857-1865, 2003. PubMed: 12750176 Schumacher A, et al. Staurosporine is a potent activator of neuronal, glial, and "CNS stem cell-like" neurosphere differentiation in murine embryonic stem cells. Mol. Cell Neurosci. 23: 669-680, 2003. PubMed: 12932446 Lee MH, et al. Simvastatin suppresses self-renewal of mouse embryonic stem cells by inhibiting RhoA geranylgeranylation. Stem Cells 25: 1654-1663, 2007. PubMed: 17464088 |