微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pAKA19 |
|---|---|
| 商品货号： | TS173880 |
| Designations： | pAKA19 |
| Depositors： | JG Coote, AK Azad |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.0000000000000000 Vector: pAKA19 (plasmid) Construction: pAKA16, pAKA18 Marker(s):ampR Construct size (kb): 5.0 Features: insert detection: lacZ marker(s): ampR other: mob replicon: pMB1 MCS: HindIII/SphI/PstI/.../NruI/HindIII |
| Applications： | integrating vector suicide vector vector permitting visual detection of recombinants vector useful for transposon mutagenesis |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; BamHI--5.0; NdeI--5.0. A suicide vector for allelic exchange or transposon mutagenesis in Pasteurella haemolytica or P. multocida. Constructed by blunt-end ligating a 485 bp HaeII fragment (from pAKA16, trimmed with T4 DNA polymerase, and containing lacZ and an MCS) to ScaI-digested pAKA18 (containing the pMB1 replicon). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Azad AK, et al. Construction of conjugative shuttle and suicide vectors for Pasteurella haemolytica and P. multocida. Gene 145: 81-85, 1994. PubMed: 8045428 |
| Shipped： | freeze-dried |