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| 产品名称： | 59B5 |
|---|---|
| 商品货号： | TS173897 |
| Organism： | Mus musculus, mouse |
| Cell Type： | embryonic stem cell |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo |
| Strain： | 129/Sv+c/+p |
| Applications： | These cell lines were used to produce mutant mice with germ line transmission for lck disruption. |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | The replacement-type vector (PmlckBSNeo2.3) was introduced into D3 embryonic stem (ES) cells by electroporation to disrupt the lck locus. Two cell lines, 59B5 (ATCC-CRL-11115) and 56B3 (ATCC-CRL-11117), were generated that were deficient for the lck gene. These cell lines were used to produce mutant mice with germ line transmission for lck disruption. Heterozygous mice were inbred to obtain mice homozygous for the disrupted lck gene. The line should be grown on feeder layers of mitomycin C treated primary mouse embryonic fibroblasts or STO cells (see ATCC 56-X.2, MITC-STO cells). A culture submitted to the ATCC as CRL-11117 in September of 1992 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. The cured cell line is available as CRL-2542. The original patent deposit is available as CRL-11117. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM 2-mercaptoethanol, 85%; fetal bovine serum, 15%
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 2 to 3 days Remove medium and rinse the monolayer with fresh 0.25% trypsin, 0.03% EDTA solution. Remove the trypsin and incubate at 37C until the cells detach (approximately 10 minutes). Add fresh medium, aspirate and dispense onto fresh feeder layer cultures. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | TW Mak |
| Deposited As： | mouse |
| U.S. Patent Number： | |
| References： | Mak TW. Mouse having a disrupted lck gene. US Patent 5,625,122 dated Apr 29 1997 |