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| 产品名称： | pFL61 |
|---|---|
| 商品货号： | TS174049 |
| Designations： | pFL61 |
| Depositors： | F Lacroute |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 5.425 DESCRIPTION OF VECTOR: Intact vector size: 5.425 Type of vector: plasmid Cloning sites: NotI Polylinker sites: EcoRI BanII SacI Asp718 KpnI AvaI SmaI XmaI BamHI XbaI Construction: pUC19 Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli Features (with orientation and position when available): marker(s): ampR, URA3 replicon: pMB1, 2 micron Vector: pFL61 (plasmid) Construction: pUC19 Marker(s):URA3,ampR Construct size (kb): 5.425 Features: marker(s): ampR, URA3 replicon: pMB1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.4; BglII--4.3, 1.1; ClaI--4.9, 0.5. A YE-type shuttle expression vector. The order of the major features in this plasmid is: EcoRI/MCS/XbaI - pGK5 - NotI - BstXI - BstXI - NotI - pGK3 - URA3 - 2 micron - pMB1 ori - AmpR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Minet M, et al. Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNA. Plant J. 2: 417-422, 1992. PubMed: 1303803 Francois Lacroute, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |