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| 产品名称： | Prototheca wickerhamii Tubaki and Soneda |
|---|---|
| 商品货号： | TS174073 |
| Strain Designations： | PR-28 UTEX 1437 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | bovine mastitis, Georgia, (?) |
| Product Format： | freeze-dried |
| Type Strain： | no |
| Medium： | ATCC® Medium 28: Emmons modification of Sabourauds agar |
| Growth Conditions： | Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 16522 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium to containing 12% sucrose to the freeze-dried inner shell vial. Once completely rehydrated, aseptically transfer the material to a 100 mm agar plate of the appropriate medium and evenly distribute the material over the surface of the agar with a spread bar. Subculture 4-6 weeks when incubated at 25C. Subculture 6-12 months when incubated at 18C To subculture, transfer a loopful of material to a fresh plate and spread evenly over the surface. |
| Subcultivation： | Protocol: ATCCNO: 16522 SPEC: This strain is distributed as a freeze-dried preparation. See the general procedures for opening a freeze-dried vial. Aseptically add 0.5 ml of ice cold medium to containing 12% sucrose to the freeze-dried inner shell vial. Once completely rehydrated, aseptically transfer the material to a 100 mm agar plate of the appropriate medium and evenly distribute the material over the surface of the agar with a spread bar. Subculture 4-6 weeks when incubated at 25C. Subculture 6-12 months when incubated at 18C To subculture, transfer a loopful of material to a fresh plate and spread evenly over the surface. |
| Name of Depositor： | WB Cooke |
| Chain of Custody： | ATCC < |
| References： | Pore RS. Prototheca taxonomy. Mycopathologia 90: 129-139, 1985. |