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微信小章| 产品名称： | pKD136 |
|---|---|
| 商品货号： | TS174262 |
| Designations： | pKD136 |
| Species： | Mus musculus, mouse |
| Depositors： | Purdue Univ., JW Frost, Purdue Univ. |
| Applications： | contains sequence produces protein 3-dehydroquinate synthase produces protein 3-dehydroshikimate dehydratase produces protein 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase produces protein protocatechuate decarboxylase produces protein transketolase |
| Insert： | DNA: genomic Gene product: 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase tkt Target Gene: , transketolase, 3-dehydroquinate synthase, protocatechuate decarboxylase, 3-dehydroshikimate dehydratase, 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase |
| Media： | ATCC Medium 1065 (see below) plus ampicillin (50.0 ug/ml) plus chloramphenicol (20.0 ug/ml) plus spectinomycin (50.0 ug/ml)
ATCC Medium 1065:
Tryptone (Difco 0123), 10.0 g
Yeast Extract (Difco 0127), 5.0 g
NaCl, 10.0 g
Distilled water, 1.0 L
|
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | A host transformed with 3 plasmids, each conferring a distinct antibiotic resistance. Recommendation for verification: EcoRI; BamHI; SphI. The tkt CDS can be recovered as a 2.4 kb EcoRI fragment. The aroF CDS can be recovered as a 5.0 kb BamHI fragment. The aroB CDS can be recovered as a 1.65 kb SphI fragment. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Draths KM, Frost JW. Genomic direction of synthesis during plasmid-based biocatalysis. J. Am. Chem. Soc. 112: 9630-9632, 1990. Draths KM, Frost JW. Synthesis using plasmid-based biocatalysis: plasmid assembly and 3-deoxy-D-arabino-heptulosonate production. J. Am. Chem. Soc. 112: 1657-1659, 1990. Draths KM, Frost JW. Environmentally compatible synthesis of adipic acid from D-glucose. J. Am. Chem. Soc. 116: 399-400, 1994. Pittard J, Wallace BJ. Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli. J. Bacteriol. 91: 1494-1508, 1966. PubMed: 5326114 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |