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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS174299 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | DG105 CMCC 2022 |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Preceptrol®： | no |
| Genotype： | thi-1 endA1 hsdR17 lacIq delta(lacZ)M15 supE44 dam-13::Tn9 F |
| Type Strain： | no |
| Comments： | Tn10 was lost spontaneously. Occasionally streak onto minimal medium and reisolate single colonies to assure M13 sensitivity. Derived from DG98 (MM294) by P1 transduction and chloramphenicol selection; isolated by D. Gelfand. A host strain for the preparation of dam- M13 DNA. |
| Medium： | ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol |
| Growth Conditions： | Temperature: 37.0°C |
| Name of Depositor： | JC Hunter-Cevera |
| References： | Lawyer FC, et al. Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J. Biol. Chem. 264: 6427-6437, 1989. PubMed: 2649500 Marinus MG, et al. Insertion mutations in the dam gene of Escherichia coli K-12. Mol. Gen. Genet. 192: 288-289, 1983. PubMed: 6316110 Jennie C Hunter-Cevera, personal communication |