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| 产品名称： | pCVD301 AD31 |
|---|---|
| 商品货号： | TS174403 |
| Designations： | pCVD301 AD31 |
| Depositors： | AR Datta |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 21.5000000000000000 Vector: pCVD301 (cosmid) Promoters: Promoter none Construction: pCVD300 and M13mp7 Marker(s):tetR Construct size (kb): 21.5 Features: marker(s): tetR promoter: none replicon: RK2 |
| Applications： | shuttle vector vector for constructing genomic libraries |
| Comments： | IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Broad host range cosmid vector which can be selected and replicate in Escherichia coli and Vibrio parahaemolyticus. |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Datta AR, et al. Shuttle cloning vectors for the marine bacterium Vibrio parahaemolyticus. J. Bacteriol. 160: 808-811, 1984. PubMed: 6094492 |
| Shipped： | freeze-dried |