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| 产品名称： | pLOI308-10 |
|---|---|
| 商品货号： | TS174449 |
| Designations： | pLOI308-10 |
| Species： | Zymomonas mobilis (Lindner) Kluyver and van Niel |
| Depositors： | University of Florida, Gainesville, LO Ingram, University of Florida, Gainesville |
| Applications： | produces protein alcohol dehydrogenase II produces protein pyruvate decarboxylase |
| Vector： | Construct size (kb): 9.0 |
| Insert： | DNA: genomic Insert lengths(kb): 6.2 Gene product: alcohol dehydrogenase II pdc Target Gene: pyruvate decarboxylase, alcohol dehydrogenase II |
| Insert Size (kb)： | 6.200 |
| Media： | 1227 plus 2% glucose
|
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI - 6.2, 2.8; BglI--2.4, 2.0, 1.4, 1.15, 0.95, 0.5, 0.3, 0.27; HindIII--3.8, 3.4, 1.65, 0.25. Expression of both enzyme activities is nearly optimal for both anaerobic and aerobic growth. The GenBank accession number for the adh insert is M15394. Constructed by inserting a promoter-less BamHI/EcoRI fragment from pLOI293 into the vector, and then inserting a promoter from Zymomonas mobilis into the BamHI site which is immediately proximal to the pdc gene. |
| References： | Ingram LO, et al. Ethanol production by Escherichia coli strains co-expressing Zymomonas pdc and adh genes. US Patent 5,000,000 dated Mar 19 1991 Conway T, et al. Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis. J. Bacteriol. 169: 2591-2597, 1987. PubMed: 3584063 Conway T, et al. Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase. J. Bacteriol. 169: 949-954, 1987. PubMed: 3029037 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |