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| 产品名称： | pMF1 |
|---|---|
| 商品货号： | TS174454 |
| Designations： | pMF1 |
| Species： | Streptococcus equisimilis Frost and Engelbrecht |
| Depositors： | University of Oklahoma, Norman, KL Hoving, University of Oklahoma, Norman |
| Applications： | produces protein streptokinase |
| Vector： | Construct size (kb): 11.80000019073486 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Streptococcus equisimilis Gene symbol: skc Gene name: streptokinase Contains complete coding sequence?: U Strain: H46A Type of DNA: genomic Insert end: HindIII Insert end: HindIII Insert size (kb): 7.4 Cross references: Insert lengths(kb): 7.400000095367432 Gene product: streptokinase skc Target Gene: streptokinase |
| Insert Size (kb)： | 7.400 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Insert contains internal sites for HindIII (3), PstI (3), SalI (2), BamHI (2), AvaI (2) and EcoRI (1). Digestion of the plasmid with PstI yields fragments of 6.0, 2.5, 2.2, 1.1 kb. Restriction digests of the clone give the following sizes (kb): PstI--5.8, 2.3, 1.9, 1.2; BamHI--8.2, 3.1; EcoRI--6.2, 5.4; BamHI/EcoRI--4.0, 3.5, 2.8, 0.5; PvuII/AvaI--4.5, 2.8, 2.2, 1.7; HindIII--4.5, 2.6 doublet, 1.9. Mucoid colony morphology on agar supplemented with ampicillin (0.5 mg/ml). The entire streptokinase (skc) structural gene is on the 2.5 kb PstI fragment. Clone expresses streptokinase of Streptococcus equisimilis which is exported into the medium. |
| References： | Ferretti JJ, Malke H. Streptokinase-coding recombinant vectors. US Patent 4,764,469 dated Aug 16 1988 Kenneth L Hoving, personal communication |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |