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微信小章| 产品名称： | Bovine viral diarrhea virus 1 |
|---|---|
| 商品货号： | TS174635 |
| Classification： | Flaviviridae, Pestivirus |
| Agent： | Bovine viral diarrhea virus 1 |
| Strain： | New York 1 |
| Common Name： | Bovine viral diarrhea virus 1 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Derived from same strain by passaging in the absence of bovine serum. Passage infected cells for optimal propagation of virus. |
| Effect on Host： | No CPE, detect by immunofluorescence or molecular-based procedures |
| Recommended Host： | BT (ATCC® CRL-1390™) |
| Growth Conditions： | Recommendations for Infection: xa0Plate cells 24-48 hours prior to infection and infect when cultures are 60% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: xa0Incubate infected culture for 4-21 days at 37°C in a humidified 5% CO2 atmosphere. |
| Name of Depositor： | M Snyder, GA Erickson, The National Animal Disease Center |
| Special Collection： | ATCC |
| Source： | Spleen from calf with diarrhea, New York, 1948 |
| References： | Baker JA. . Am. J. Vet. Res. 15: 525, 1954. Kim SG, Dubovi EJ. A novel one-step single-tube RT-duplex PCR method with an internal control for detection of bovine viral diarrhea virus in bulk milk, blood, and follicular fluid samples. Biologicals. 31: 103-106, 2003. PubMed: 12770539 |