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| 产品名称： | pFM23 |
|---|---|
| 商品货号： | TS174728 |
| Designations： | pFM23 |
| GenBank Number： | M33002 |
| Species： | tobacco necrosis necrovirus |
| Depositors： | F Meulewaeter |
| Vector： | Construct size (kb): 3.660000085830689 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.9179999828338623 Gene product: coat protein, 30 kDa ORF5 |
| Insert Size (kb)： | 0.918 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--2.7, 0.9; EcoRI--3.1, 0.6; BamHI--3.6. The insert contains the following restriction sites (approximate kb from the 5 end): NcoI--0.48, 0.66; EcoRI--0.6; KpnI--0.65; XmnI--0.72; BamHI--0.91. The plasmid is not stable in E. coli MC1061. Corresponds to nt 2593-3510 of the virus, including the complete coding sequence for the coat protein. Transcription from the T7 promoter gives the sense (+) strand. |
| Classification： | Necrovirus |
| References： | Meulewaeter F, et al. Genome structure of tobacco necrosis virus strain A. Virology 177: 699-709, 1990. PubMed: 2371773 Frank Meulewaeter, personal communication |