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| 产品名称： | Chondrocyte Differentiation Tool | |||||||||
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| 商品货号： | TS174784 | |||||||||
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
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| Product Format： | frozen 100 mL | |||||||||
| Storage Conditions： | -20°C (or -70°C for long-term storage) | |||||||||
| Comments： | The Chondrocyte Differentiation Tool is a complete differentiation medium designed to induce chondrogenesis in actively proliferating Adipose-Derived Mesenchymal Stem Cells with high efficiency. The Chondrocyte Differentiation Tool provides enough medium to differentiate up to ~ 13.5 x 107 cells, sufficient to seed 20 wells of a 48-well tissue culture plate when using alginate encapsulation. Chondrocyte differentiation requires that the cells be grown in a three-dimensional aggregate cell culture. Micromass culture can be used, but for the best results, ATCC recommends the use of a matrix, such as alginate, to provide a scaffold for the deposition of proteoglycans. Detailed instructions for the formation of chondrogenic microbeads are provided. |
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| Complete Growth Medium： | Antimicrobials and phenol red are not required but may be added to the Chondrocyte Differentiation Tool if desired prior to use. The recommended volume of each optional component to be added to Chondrocyte Differentiation Tool is summarized in Table 1. xa0 Table 1. Optional Addition of Antimicrobials/Antimycotics and Phenol Red per 100 mL of Medium
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