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| 产品名称： | pTrcrham-15 plasmid in E. coli |
|---|---|
| 商品货号： | TS174830 |
| Designations： | pTrcrham-15 plasmid in E. coli |
| GenBank Number： | X60472 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | C Wong |
| Applications： | produces protein rhamnulose-1-phosphate aldolase |
| Vector： | Construct size (kb): 5.185 |
| Insert： | DNA: genomic Insert lengths(kb): 0.82 Gene product: rhamnulose-1-phosphate aldolase rhaD Target Gene: rhamnulose-1-phosphate aldolase |
| Insert Size (kb)： | 0.822 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--4.4, 0.82; EcoRI--uncut; EcoRV--4.2, 0.9. This construct expresses the gene in E. coli. The expressed protein contains 36 extra amino acids at the N-terminus which can be removed by enterokinase digestion. The protein is still functional without removing these extra amino acids. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Chi-Huey Wong, personal communication |