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微信小章| 产品名称： | hGR(1-777) |
|---|---|
| 商品货号： | TS174970 |
| Designations： | hGR(1-777) |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein glucocorticoid receptor |
| Vector： | Construct size (kb): 8.90000057220459 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pKCR-2 Intact vector size: 5.700 Type of vector: plasmid Vector end: BamHI Vector end: BamHI Cloning sites: BamHI, EcoRI Polylinker sites: Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR promoter: SV40 replicon: pMB1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: GRL Genomic copy number: unique Gene name: glucocorticoid receptor Contains complete coding sequence?: Y Chromosome: 5; Localization: 5 q31-q32 Tissue: breast adenocarcinoma MCF7 cell line Type of DNA: cDNA Insert end: Modification: BamHI linkers Insert end: Modification: BamHI linkers Insert size (kb): 2.88 Cross references: Insert lengths(kb): 2.880000114440918 Tissue: breast adenocarcinoma MCF7 cell line Gene product: glucocorticoid receptor GRL Target Gene: glucocorticoid receptor |
| Insert Size (kb)： | 2.880 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.2, 3.1; EcoRI--5.8, 1.8, 0.86, 0.78; KpnI--9.4; XbaI--9.4; PstI--6.8, 2.2. The orientation of the insert is: 5 BamHI, SmaI, KpnI, SacI, EcoRI, coding region for amino acids 1-777, XbaI/BamHI 3. Cross hybridizes with a 6.8 kb rat liver mRNA. pKCR-2 is an expression vector containing SV40 early and late promoters and beta-globin splicing and polyadenylation signals. Constructed from overlapping cDNA clones. |
| References： | LeClerc S, et al. Molecular Cloning and characterization of a factor that binds the human glucocorticoid receptor gene and represses its expression. J. Biol. Chem. 266: 17333-17340, 1991. PubMed: 1894621 Green S, Chambon P. Oestradiol induction of a glucocorticoid-responsive gene by a chimaeric receptor. Nature 325: 75-78, 1987. PubMed: 3025750 Govindan MV, et al. Cloning of the human glucocorticoid receptor cDNA. Nucleic Acids Res. 13: 8293-8304, 1985. PubMed: 2417195 Govindan MV. Specific region in hormone binding domain is essential for hormone binding and trans-activation by human androgen receptor. Mol. Endocrinol. 4: 417-427, 1990. PubMed: 2342476 Govindan MV, et al. Differential regulation of mouse mammary tumor virus-bacterial chloramphenicol acetyltransferase chimeric gene by human mineralocorticoid hormone-receptor complexes. J. Steroid Biochem. Mol. Biol. 39: 91-103, 1991. PubMed: 1648951 Govindan MV, et al. Human glucocorticoid receptor gene promoter-homologous down regulation. J. Steroid Biochem. Mol. Biol. 40: 317-323, 1991. PubMed: 1958537 Leclerc S, et al. Purification of a human glucocorticoid receptor gene promoter-binding protein. J. Biol. Chem. 266: 8711-8719, 1991. PubMed: 2026589 |