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| 产品名称： | pHKHK3a |
|---|---|
| 商品货号： | TS174996 |
| Designations： | pHKHK3a |
| GenBank Number： | X78678 |
| Species： | Homo sapiens, human |
| Depositors： | DT Bonthron |
| Applications： | The second polyadenylation signal was used. |
| Vector： | Construct size (kb): 6.400000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pCDM8 Intact vector size: 4.356 Type of vector: phagemid Vector end: BstXI Vector end: BstXI Cloning sites: HindIII XbaI XhoI BstXI PstI NotI Polylinker sites: HindIII XbaI XhoI BstXI --stuffer--BstXI XhoI PstI NotI XbaI Construction: CMV, SV40 Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): replicon: pMB1, 1-587 replicon: M13, marker(s): supF, promoter: CMV, ->, 1516-2170 promoter for in vitro transcription: T7, ->, 2171-2189 MCS: HindIII...XbaI, ->, 2198-2655 terminator: SV40 splice site and polyadenylation, ->, 2656-3352 replicon: polyoma, 3353-4188 replicon: SV40, 4189-4352 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: KHK Gene name: ketohexokinase (fructokinase) Contains complete coding sequence?: Y Tissue: Hep G2 cell line Disease: hepatocellular carcinoma Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 1.899 Cross references: DNA Seq. Acc.: X78678 Nucleotides 1-1899 of the insert correspond to nucleotides 1-1899 of X78678. Insert lengths(kb): 1.89900004863739 Tissue: Hep G2 cell line hepatocellular carcinoma Disease: hepatocellular carcinoma Gene product: ketohexokinase (fructokinase) KHK |
| Insert Size (kb)： | 1.899 |
| Media： | ATCC® Medium 1951: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 15 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XbaI--4.3, 2.1; HindIII--6.4; BamHI--3.9, 2.4; SacI--4.7, 0.87, 0.7; PstI--4.7, 1.1, 0.5. The insert contains the following restriction sites (approximate kb from the 5 end): BamHI--0.41, SacI--0.54, 1.36; PstI--0.46, 0.88. Derived from the form A variant transcript (alternately spliced first coding exon). The second polyadenylation signal was used. |
| References： | Bonthron DT, et al. Molecular basis of essential fructosuria: molecular cloning and mutational analysis of human ketohexokinase (fructokinase). Hum. Mol. Genet. 3: 1627-1631, 1994. PubMed: 7833921 |