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| 产品名称： | pHG276 |
|---|---|
| 商品货号： | TS175006 |
| Designations： | pHG276 |
| Depositors： | GD Stewart |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.3200001716613770 Vector: pHG276 (plasmid) Promoters: Promoter lambda PR Construction: pHG165, lambdaPR Marker(s):ampR Construct size (kb): 4.320000171661377 Features: insert detection: lacZ marker(s): ampR promoter: lambda PR replicon: pMB1 repressor gene: cI857 |
| Applications： | expression vector vector permitting construction of fusion proteins vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HpaI--3.0, 1.3. Plasmid expression vector with lambda PR promoter, plasmid copy number controlled by rop(rom), and cI857 for temperature regulation of lac and insert expression. A 1.7 kb BamHI/HpaI fragment containing the cI gene and lambda PR was cloned into BamHI/HpaI partially digested pHG165 (ATCC 37618). |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Stewart GS, et al. pHG276: a multiple cloning site pBR322 copy number vector expressing a functional lacalpha peptide from the bacteriophage lambda PR promoter. Plasmid 15: 182-190, 1986. PubMed: 3012612 |
| Shipped： | freeze-dried |