微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pAPPA1 plasmid in E. coli |
|---|---|
| 商品货号： | TS175039 |
| Designations： | pAPPA1 plasmid in E. coli |
| GenBank Number： | M58708 |
| Species： | Escherichia coli (Migula) Castellani and Chalmers |
| Depositors： | RL Van Etten |
| Applications： | produces protein acid phosphatase, pH 2.5, exopolyphosphatase/phytase |
| Vector： | Construct size (kb): 3.75 |
| Insert： | DNA: genomic Insert lengths(kb): 1.3 Gene product: acid phosphatase, pH 2.5, exopolyphosphatase/phytase appA* Target Gene: acid phosphatase, pH 2.5, exopolyphosphatase/phytase |
| Insert Size (kb)： | 1.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI--3.9; SalI--2.8, 1.1; XbaI--2.3, 1.4. The insert contains the following restriction sites (approximate kb from the 5 end): BglI--0.18, 0.37; SalI--0.34; SacI--1.11. Digestion with XbaI yields an intact insert with a portion of the T7 phi 10 promoter included at the 5 end and a portion of the MCS at the 3 end. Extraneous 3 material can be removed with an EcoRI digest. The pH 2.5 acid phosphatase is more active as a phytase. |
| Classification： | Enterobacteriaceae, Escherichia |
| References： | Ostanin K, et al. Overexpression, site-directed mutagenesis, and mechanism of Escherichia coli acid phosphatase. J. Biol. Chem. 267: 22830-22836, 1992. PubMed: 1429631 Robert L Van Etten, personal communication |