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微信小章| 产品名称： | 9-2-4 |
|---|---|
| 商品货号： | TS175090 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody binds specifically to an idiotypic determinant on TC-31 anti-exotoxin A monoclonal antibody. |
| Storage Conditions： | liqiud nitrogen vapor phase |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against an idiotypic determinant on TC-31 monoclonal antibody (against exotoxin A) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against an idiotypic determinant on TC-31 monoclonal antibody (against exotoxin A) |
| Comments： | Animals were immunized with TC-31 (ATCC CRL-2494), a monoclonal antibody to exotoxin A of Pseudomonas aeruginosa. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody binds specifically to an idiotypic determinant on TC-31 anti-exotoxin A monoclonal antibody. Exotoxin A inhibits the binding of the 9-2-4 antibody to TC-31. This antibody conjugated to keyhole limpet hemocyanin (KLH) can induce antibodies in mice that bind to exotoxin A and protect 80% of the mice from a lethal injection of exotoxin A. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 4 to 5 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 5 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal:xa0Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | mouse IgG1 |
| Name of Depositor： | DG Sieckmann |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| Year of Origin： | 1986 |
| References： | Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |