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微信小章| 产品名称： | pBRZ |
|---|---|
| 商品货号： | TS175103 |
| Designations： | pBRZ |
| Species： | Homo sapiens, human |
| Applications： | A 1.16 kb BglII/PvuII fragment which includes parts of exon 1 and intron 1 (IVS-1 probe) has been used to detect the SacI and PvuII polymorphisms. A 1.8 kb SacI fragment has been used to detect XbaI and PvuII polymorphisms. |
| Vector： | Construct size (kb): 9.0 |
| Insert： | DNA: genomic Insert lengths(kb): 5.0 Gene product: hemoglobin, zeta HBZ Alleles: A1, A2, C1, C2, C3, C4, G1, G2, |
| Insert Size (kb)： | 5.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--9.0; BamHI--9.0; EcoRI/BamHI--4.75, 4.1; HindIII--uncut; PstI--3.3, 1.4, 1.3, 1.05 + others. This insert contains the complete zeta globin pseudogene 1 with approximately 1.5 kb flanking sequence upstream and 1.1 kb flanking sequence downstream. A 1.16 kb BglII/PvuII fragment which includes parts of exon 1 and intron 1 (IVS-1 probe) has been used to detect the SacI and PvuII polymorphisms. A 1.8 kb SacI fragment has been used to detect XbaI and PvuII polymorphisms. The 5 EcoRI end of the insert was engineered during construction of the probe. |
| References： | Hill AV, et al. Recombination within the human embryonic zeta-globin locus: a common zeta-zeta chromosome produced by gene conversion of the psi zeta gene. Cell 42: 809-819, 1985. PubMed: 2996777 Higgs DR, et al. Analysis of the human alpha-globin gene cluster reveals a highly informative genetic locus. Proc. Natl. Acad. Sci. USA 83: 5165-5169, 1986. PubMed: 3014536 Goodbourn SE, et al. Molecular basis of length polymorphism in the human zeta-globin gene complex. Proc. Natl. Acad. Sci. USA 80: 5022-5026, 1983. PubMed: 6308667 |