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产品简介
购买须知
| 产品名称： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
|---|---|
| 商品货号： | TS175193 |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| Strain Designations： | H4S47C |
| Alternate State： | Candida robusta Diddens et Lodder |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Type Strain： | no |
| Genotype： | MATa, his3delta1, leu2delta0, met15delta0, URA3 |
| Preceptrol®： | no |
| Mating Type： | a |
| Comments： | Temperature sensitive HHF1 modfied to H4S47C via site directed mutagenesis. The second histone H4 gene, HHF2, was knocked out and replaced with the URA3 selection marker. |
| Morphology： | On YEPD medium at 25°C after 5 days cells are globose, ovoidal or elongate and are usually isolated or in small groups. Growth is butyrous and light cream colored. |
| Medium： | ATCC® Medium 1069: YPAD medium ATCC® Medium 1245: YEPD ATCC® Medium 200: YM agar or YM broth |
| Growth Conditions： | Temperature: 20°C to 25°C Atmosphere: Typical aerobic |
| Name of Depositor： | K Brogaard, J Widom |
| Special Collection： | ATCC |
| Chain of Custody： | ATCC <-- K Brogaard, J Widom |
| Isolation： | Targeted mutagenesis from BY4741. |
| References： | Brogaard K, et al. A map of nucleosome positions in yeast at base-pair resolution. Nature 486(7404): 496-501, 2012. PubMed: 22722846 |