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| 产品名称： | pFM24 |
|---|---|
| 商品货号： | TS175269 |
| Designations： | pFM24 |
| GenBank Number： | M33002 |
| Species： | tobacco necrosis necrovirus |
| Depositors： | F Meulewaeter |
| Vector： | Construct size (kb): 4.360000133514404 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.613999962806702 Gene product: ORF1, 23 kDa ORF1 |
| Insert Size (kb)： | 1.614 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--2.8, 1.7; XhoI--4.4; KpnI--3.1, 1.25. The insert contains the following restriction sites (approximate kb from the 5 end): PstI--0.15; BglII--0.16; SpeI--0.97; KpnI--1.18; XhoI--1.37. The plasmid is not stable in E. coli MC1061. Includes nt 19-1632 of the virus, including the complete ORF1 and part of ORF2. Transcription from the T7 promoter gives the sense (+) strand. |
| Classification： | Necrovirus |
| References： | Meulewaeter F, et al. Genome structure of tobacco necrosis virus strain A. Virology 177: 699-709, 1990. PubMed: 2371773 Frank Meulewaeter, personal communication |