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| 产品名称： | Pellita catalonica |
|---|---|
| 商品货号： | TS175478 |
| Deposited As： | Pardalotamoeba marina |
| Strain Designations： | CON-1 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Marine invertebrate Connecticut, United States Isolation date: August 4, 2002 |
| Product Format： | frozen |
| Type Strain： | no |
| Comments： | Originally deposited as Pardalotamoeba marina. Name changed by depositors request. |
| Medium： | ATCC® Medium 1525: Seawater 802 medium |
| Growth Conditions： | ATCC medium 1525: Seawater 802 medium Temperature: 20.0°C Grown with Klebsiella pneumoniae subsp. pneumoniae ATCC 700831 |
| Subcultivation： | Protocol: Scrape the bottom of the flask, agitate quickly, and aseptically withdraw 0.2 ml of cell suspension. Inoculate in a T25 flask containing 10 ml of ATCC medium 1525 bacterized with Klebsiella pneumoniae subsp. pneumoniae ATCC 700831. Note: The amoebae settle very quickly so the transfer must be done rapidly. |
| Cryopreservation： | Harvest cells 2 to 3 days post-peak density. Decant the culture fluid leaving approximately 1 ml to cover the attached cells. Prepare a 20% DMSO solution using the decanted fluid. Detach cells using a sterile cell scraper and determine the volume of the concentrated cell suspension. Add an equal volume of the 20% DMSO solution, yielding a final concentration of 10%. Aliquot the suspension in 0.5-ml amounts to screw-capped 1.2-ml Nalgene cryovials and transfer them to a Nalgene 5100 Cryo 1 C Freezing Container ("Mr. Frosty"). After 3 hours at -80 C, rapidly plunge the vials into liquid nitrogen.
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| Name of Depositor： | TA Nerad, JC Cole |
| Special Collection： | NSF - Protistology |
| Year of Origin： | August 4, 2002 |
| References： | carapace of an American lobster, Homarus americanus, Connecticut Thomas A Nerad, personal communication Smirnov AV, Kudryavtsev AA. Pellitidae n. Fam. (Lobosea, Gymnamoebia) - a new family, accommodating two amoebae with an unusual cell coat and an original mode of locomotion, Pellita catalonica n.g., n.sp. and Pellita digitata comb. nov. Eur. J. Protistol. 41: 257-267, 2005. |