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| 产品名称： | pSE103 |
|---|---|
| 商品货号： | TS175514 |
| Designations： | pSE103 |
| Depositors： | SJ Elledge |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 0E-16 Vector: pSE103 (plasmid) Construction: pSE101, lambda cI857 Marker(s):kanR Construct size (kb): 0.0 Features: marker(s): kanR replicon: pSC101 repressor gene: cI857 |
| Applications： | vector with low copy number |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--13.0; HindIII--9.4, 3.3; PstI--10.4, 1.7, 0.9. This plasmid vector has lambda cI857 and a pSC101 replicon. It is compatible with the pDPT427-derived replicon as well as with the ColE1 replicon. BglII-cleaved lambda cI857 was ligated into the BamHI site of pSE101. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Elledge SJ, Walker GC. Phasmid vectors for identification of genes by complementation of Escherichia coli mutants. J. Bacteriol. 162: 777-783, 1985. PubMed: 2985547 |
| Shipped： | freeze-dried |