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| 产品名称： | p424 GAL1 |
|---|---|
| 商品货号： | TS175515 |
| Designations： | p424 GAL1 |
| Depositors： | M Funk |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 6.3070001602172850 DESCRIPTION OF VECTOR: Intact vector size: 6.307 Type of vector: plasmid Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI Construction: pRS424, GAL1 promoter Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): TRP1, ->, 200-1213 terminator: CYC1, ->, 1895-2155 MCS: XhoI...SpeI, ->, 2155-2212 promoter for expression: GAL1, marker(s): ampR, replicon: 2 micron, ->, 4860-6200 Vector: p424 GAL1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pRS424, GAL1 promoter Marker(s):TRP1,ampR Construct size (kb): 6.307000160217285 Features: marker(s): TRP1 marker(s): ampR promoter for expression: GAL1 replicon: 2 micron MCS: XhoI...SpeI terminator: CYC1 |
| Applications： | YE-type (episomal) shuttle vector YX-type (expression) shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 1.7, 1.0, 0.45; EcoRI--6.3; BamHI--6.3. Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736 |
| Related Products： | component of:ATCC 87670 |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |