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| 产品名称： | Hf32 |
|---|---|
| 商品货号： | TS175533 |
| Designations： | Hf32 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 6.5 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.099999904632568 Tissue: skin fibroblast Gene product: collagen, type I, alpha 2 COL1A2 Alleles: A1, B1, K1, C1, A2, C2, K2, b2 |
| Insert Size (kb)： | 2.100 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The 5 EcoRI site was lost in cloning. The insert contains the following sites (in approximate kb from the 5 end): AvaI--0.3; PvuII--0.5, XhoI--0.75; AvaI--0.8; PstI--1.15; BglI-1.35; XbaI--1.55; SacI--1.85. Restriction digests of the clone give the following sizes (kb): HindIII--6.5; EcoRI--6.5; PstI--4.6, 1.8; BamHI--6.5; SalI--6.5; HindIII/SstI--4.2, 1.2, 1.1. This insert codes for 480 residues of the collagenous region. It also encodes most of the COOH-terminal propeptide. This clone detects major mRNA species of about 5.1 and 4.6 kb in human skin fibroblasts and a minor transcript of about 4.4 kb. |
| References： | Myers JC, et al. Cloning a cDNA for the pro-alpha2 chain of human type I collagen. Proc. Natl. Acad. Sci. USA 78: 3516-3520, 1981. PubMed: 6267597 Bernard MP, et al. Structure of a cDNA for the proalpha2 chain of human type I procollagen. Comparison with chick cDNA for proalpha2(I) identifies structurally conserved features of the protein and the gene. Biochemistry 22: 1139-1145, 1983. PubMed: 6687691 |