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| 产品名称： | pLB1 |
|---|---|
| 商品货号： | TS175588 |
| Designations： | pLB1 |
| Species： | Azotobacter vinelandii Lipman |
| Depositors： | PE Bishop |
| Vector： | Construct size (kb): 8.600000381469727 |
| Insert： | DNA: genomic Insert lengths(kb): 2.599999904632568 Gene product: dinitrogenase reductase nifK |
| Insert Size (kb)： | 2.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Insert contains PstI, HindIII, AvaI and SalI sites. The insert contains the following sites separated by (bp)(approx): EcoRI- 175- PstI, HindIII- 520- PstI- 175- AvaI- 300- PstI- 175- SalI- 175- SalI- 910- SalI- 130- EcoRI. This is a clone of the nifK gene, including the wild type alleles for nif-6 and nif-38. |
| References： | Bishop PE, et al. Molecular cloning of nif DNA from Azotobacter vinelandii. J. Bacteriol. 162: 21-28, 1985. PubMed: 3884589 |