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| 产品名称： | cRH15A |
|---|---|
| 商品货号： | TS175611 |
| Designations： | cRH15A |
| Species： | Homo sapiens, human |
| Applications： | The whole cosmid has been used as the probe. |
| Vector： | DESCRIPTION OF VECTOR COMPONENT: Name of vector: unknown Intact vector size: Type of vector: cosmid Cloning sites: Polylinker sites: Host range: Escherichia coli Features (with orientation and position when available): marker(s): ampR Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: LDLR Genomic copy number: unique Gene name: low density lipoprotein receptor (familial hypercholesterolemia) Contains complete coding sequence?: U Chromosome: 19; Localization: 19 p13.2 Type of DNA: genomic Insert end: Insert end: Insert size (kb): 40.0 Cross references: Insert lengths(kb): 40.0 Gene product: low density lipoprotein receptor (familial hypercholesterolemia)(low density lipoprotein receptor) LDLR Alleles: A2, E1, E2, A1, A2, B1, B2, D1, D2, K1, K2, Q1, Q2, S2, A1, S1 |
| Insert Size (kb)： | 40.0 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 9.0, 8.4, 3.8, 3.1, 2.0, 1.1, 0.98; BglII--15.0 (doublet), 6.0, 5.2, 4.4, 1.6, 1.3; EcoRI--15.0, 12.0, 9.4, 8.8, 2.3, 1.4; HindIII--19.0, 15.0, 8.2, 3.4, 2.5; BstEII--11.0, 9.4, 8.4 (doublet), 5.4, 4.5, 2.3. The whole cosmid has been used as the probe. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzymes not detecting polymorphism: BglI, BstEII, BxtXI, DraI, EcoRV, HincII, XmnI, MspI, TaqI, BamHI, BglII, PstI, EcoRI, HindIII. Polymorphisms were also not detected with SacI or KpnI in those individuals demonstrating polymorphism with SacI/KpnI-digested DNA. |
| References： | Hegele RA, et al. Three RFLPs upstream of the low density lipoprotein receptor (LDLR) gene. Nucleic Acids Res. 17: 470, 1989. PubMed: 2563158 |