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| 产品名称： | pUK21deltaBB |
|---|---|
| 商品货号： | TS175654 |
| Designations： | pUK21deltaBB |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 |
| Vector Information： | Size (kb): 3.0000000000000000 DESCRIPTION OF VECTOR: Intact vector size: 3.000 Type of vector: plasmid Cloning sites: StuI XhoI EcoRV NotI XbaI ClaI Polylinker sites: SpeI StuI XhoI EcoRV NotI XbaI SpeI ClaI Construction: pUC Features (with orientation and position when available): Vector: pUK21deltaBB (plasmid) Construction: pUC21 Marker(s):kanR Construct size (kb): 3.0 |
| Applications： | cloning vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRV--3.0; PvuI--1.7, 1.3; XbaI--3.0. The plasmid derived from pUK21 described by deleting the BamHI-BglII fragment. |
| Media： | ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C Protocol: (LB + kan) |
| References： | Vieira J. New pUC-derived cloning vectors with different selectable markers and DNA replication origins. Gene 100: 189-194, 1991. PubMed: 1905257 Voth WP. Yeast vectors for integration at the HO locus.. Nucleic Acids Res. 29(12): E59-9, 2001. PubMed: 11410682 David J Stillman, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |